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The primers were designed to Rv1510 gene. Ninety nine samples of M. tuberculosis and sixty samples of M. bovis were tested and no sample of the bovine bacillus was detected by the qPCR. Statistical tests showed no difference between the qPCR and biochemical tests used to identify the Mycobacterium tuberculosis. The correlation between tests was perfect with Kappa index of 1.0 (p < 0.001, CI = 0.84 …
Cited by: 5Robustness of the PCR assay was not assessed in a formal validation but was evaluated retrospectively after method development. Some of the parameters assessed were variations in the extraction method, the extraction temperature, the touchdown profile, and the amount of DNA in the PCR.
Cited by: 12Aug 01, 2014 · In Japan, the estimated number of patients with type 2 diabetes has increased to 10.7 million, the sixth largest such population in the world (16). However, there were no human studies on gut microbiota composition in Japanese patients with type 2 diabetes.
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Jan 20, 2020 · Inflammatory bowel diseases (IBD) are associated with alterations in gut microbial abundances and lumenal metabolite concentrations, but the effects of …
Author: Nadine Fornelos, Eric A. Franzosa, Eric A. Franzosa, Jason Bishai, John W. Annand, Akihiko Oka, Jaso...Independent (holdout/external) validation consisted of two steps: (1) application of the feature filtering and normalization using the same parameters as for cross-validation data (i.e., discarding features according to the cross-validation low-abundance filter and applying the log-standardization with mean and standard deviation values as ...
Cited by: 418Incubate portions of the media for 14 days. No growth of microorganisms occurs. Growth promotion test of aerobes, anaerobes and fungi. Test each batch of ready-prepared medium and each batch of medium prepared either from dehydrated medium or from ingredients. Suitable strains of microorganisms are indicated in Table 1.
File Size: 396KBJun 25, 2010 · Importantly, the outer layers of the masks, which contain ∼2.2% copper oxide particles, did not cause any skin sensitization or skin irritation as determined in animal studies (data not shown). Also similar fabrics containing 6 times higher amounts of …
Cited by: 120Jan 23, 2018 · The N95 respirator is the most common of the seven types of particulate filtering facepiece respirators. This product filters at least 95% of airborne particles but is not resistant to oil. This web page provides a table of NIOSH-approved N95 respirators, listed alphabetically by …
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Feb 05, 2018 · Advances in synthetic biology allow the generation of ever more sophisticated engineered bacteria. In this Review, Riglar and Silver showcase recent highlights in …
Cited by: 55This is the first randomized study to test whether the performance of salivary strips is affected by the use of mouthwash. • Salivary NO 2 − strips have a high level of reproducibility and repeatability in detecting changes in salivary NO 2 −.. Salivary strips are not sufficiently accurate for the measurement of absolute concentrations of NO 2 − in saliva.
Author: Abrar M. Babateen, Abrar M. Babateen, Oliver M. Shannon, John C. Mathers, Mario Siervo, Mario SiervoAug 07, 2015 · World Health Organization International Standard To Harmonize Assays for Detection of Mycoplasma DNA C. Micha Nübling , a Sally A. Baylis , a Kay-Martin Hanschmann , a Thomas Montag-Lessing , a, † Michael Chudy , a Julia Kreß , a Ursula Ulrych , b Stefan Czurda , b, * Renate Rosengarten , b, c, * and the Mycoplasma Collaborative Study Group
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Sep 18, 2016 · No bacteria or viruses were detected on artificially contaminated endoscopes after a 5-minute exposure to superoxidized water 551 and HBV-DNA was not detected from any endoscope experimentally contaminated with HBV-positive mixed sera after a disinfectant exposure time of 7 minutes 552. Top of Page. Uses.
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The red line illustrates the number of key bacteria in the discovery set. B, The relative abundance of each bacteria in the predictive model was assessed using the mean decrease accuracy in 27 datasets. The red line illustrates five key bacteria filtered by random forest method via fivefold cross‐validation.
Dec 12, 2019 · Rapid identification and characterisation of drug-resistant bacterial pathogens have an important role in diagnostic and antimicrobial stewardship. Response time in …
Author: Samantha Flores-Treviño, Elvira Garza-González, Soraya Mendoza-Olazarán, Rayo Morfín-Otero, Adrián C...Is your water safe? A new standard can help safeguard against a Legionella outbreak. It can lurk in your cooling towers, piping systems, showerheads, hot tubs, sauna rooms, and decorative fountains. It causes flu-like symptoms, hospitalizations, lingering neurological issues, and death. Legionella bacteria begin as a microscopic presence in your water systems and can balloon into a tarnished ...
May 01, 2010 · Implementing a rapid test method to replace currently accepted methodology for mycoplasma detection requires overcoming unique validation challenges specific to these organisms. Rapid microbiological methods (RMM) designed to detect bacteria and fungi are challenging to validate as replacements for compendial methods in general. As a result, using RMM to release finished …
Author: John DuguidEvaluating the efficacy of cloth facemasks in reducing particulate matter exposure Article in Journal of Exposure Science and Environmental Epidemiology 27(3) · August 2016 with 1,007 Reads
G9a Is Essential for Epigenetic Silencing of K + Channel Genes in Acute-to-Chronic Pain Transition Geoffroy Laumet , 1 Judit Garriga , 2 Shao-Rui Chen , 1 Yuhao Zhang , 1 De-Pei Li , 1 Trevor M. Smith , 1 Yingchun Dong , 1, 3 Jaroslav Jelinek , 2 Matteo Cesaroni , 2 Jean-Pierre Issa , 2 and Hui-Lin Pan 1
Cited by: 78Oct 04, 2019 · Gut bacteria were significantly more diverse ... Japan), which is an authorized medical device in Japan (No. 22500BZX00522000). ... a relative validation …
Cited by: 2Aspiration or ingestion of these bacteria might be associated with poorer outcome of allo-HSCT recipients. However, the major cause of mortality was progressive diseases, and no patient died of the complications associated with detected Staphylococcus haemolyticus or Ralstonia pickettii in the present study. The incidence of bacteremia until ...
The ability of the activated carbon, silver impregnated activated carbon, and silica sand to eliminate and destroy water borne E. coli BL21 were tested under plate assay and shake flask technique. Silver nanoparticles showed the highest antibacterial effect against E. …
Apr 05, 2007 · The ability to monitor a pathogen's gene expression program in response to the host environment is central to understanding host–microbe interactions. This …
Cited by: 69Oct 28, 2019 · In Japan, an ideal bodyweight (IBW) calculated by 22 × height (m) 2 has commonly been used in the planning of medical nutrition therapy (MNT). However, there have been concerns regarding calorie deficits in fulfilling resting energy expenditure (REE) for patients with type 2 diabetes undergoing MNT as defined by 25 kcal/kg IBW/day.
Author: Takuya Fukuda, Muhei Tanaka, Masahiro Yamazaki, Yoshinori Marunaka, Yoshinori Marunaka, Michiaki Fuk...Aspiration or ingestion of these bacteria might be associated with poorer outcome of allo-HSCT recipients. However, the major cause of mortality was progressive diseases, and no patient died of the complications associated with detected Staphylococcus haemolyticus or Ralstonia pickettii in the present study. The incidence of bacteremia until ...
Validation of a loop-mediated isothermal amplification assay forrapiddiagnosisofpertussis ... Tokyo, Japan) was used for primer design. Five μl of primers F3/B3 (0.2 µM), FIP/BIP (2 µM) and ... rest of listed bacteria in Table 1 were retrieved from our clinical
Moringa oleifera leaf (MOL) has been proved to improve silage fermentation but the exact reason is unclear yet.Fermentation quality, protein preservation, antioxidant activity and bacterial community of stylo and alfalfa ensiled with 5%, 10% dry MOL, stylo or alfalfa were investigated.
tion preparation manufacturing. As the most standard method of microbial control for pharmaceutical water, the Japanese Pharmacopoeia specifi es a method that involves fi ltering the pharmaceutical water with a fl at fi lter, then culturing it for 4 to 7 days on one or two types of agar culture media (the fi lter membrane method).
This is the first randomized study to test whether the performance of salivary strips is affected by the use of mouthwash. • Salivary NO 2 − strips have a high level of reproducibility and repeatability in detecting changes in salivary NO 2 −.. Salivary strips are not sufficiently accurate for the measurement of absolute concentrations of NO 2 − in saliva.
A fully integrated bacterial detection system based on ‘count-on-a-cartridge (COC)’ platform is devloped. • Culture-free, rapid, accurate assay protocol within a couple of hours is …
Abstract. A yeast (Saccharomyces cerevisiae) DNA repair reporter assay termed the GreenScreen ® assay (GSA) is described.This is a novel, cost‐effective genotoxicity screen, developed to provide a pre‐regulatory screening assay for use by the pharmaceutical industry and in other applications where significant numbers of compounds need to be tested.
Gut bacteria were significantly more diverse ... Japan), which is an authorized medical device in Japan (No. 22500BZX00522000). ... a relative validation of the test-version by comparison with 3 ...
There are twenty-eight OSHA-approved State Plans, operating state-wide occupational safety and health programs. State Plans are required to have standards and enforcement programs that are at least as effective as OSHA's and may have different or more stringent requirements.
Abstract. 16S rRNA gene-based analysis of rumen Prevotella was carried out to estimate the diversity and diet specificity of bacteria belonging to this genus. Total DNA was extracted from the rumen digesta of three sheep fed two diets with different hay-to-concentrate ratios (10 : 1 and 1 : 2).
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Salmonella enterica causes an estimated 1 million illnesses in the United States each year, resulting in 19,000 hospitalizations and 380 deaths, and is one of the four major global causes of ...
Quinol-dependent nitric oxide reductases (qNORs) are membrane-integrated, iron-containing enzymes of the denitrification pathway, which catalyze the reduction of nitric oxide (NO) to the major ozone destroying gas nitrous oxide (N2O). Cryo–electron microscopy structures of active qNOR from Alcaligenes xylosoxidans and an activity-enhancing mutant have been determined to be at local ...
Feb 12, 2020 · Immune checkpoint inhibitors are a promising and increasingly popular approach to cancer therapy. Unfortunately, they sometimes have severe immune-mediated side effects, which can be minimized by local delivery of immunotherapy to the tumors. To address this problem, Gurbatri et al. engineered a probiotic bacteria system to release nanobodies targeting the immune checkpoints. …
Author summary Throughout evolution, bacteria were exposed to reactive oxygen species and evolved the ability to scavenge toxic oxygen radicals. Furthermore, multicellular organisms evolved the ability to produce such oxygen species directed against pathogens. Recent studies also suggest that ROS such as H2O2 play an important role during host gut colonisation by its microbiota. Traditionally ...
ATCC stands ready to support our customers’ needs during the coronavirus pandemic. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services.
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Objective There is a need for an improved biomarker for colorectal cancer (CRC) and advanced adenoma. We evaluated faecal microbial markers for clinical use in detecting CRC and advanced adenoma. Design We measured relative abundance of Fusobacterium nucleatum ( Fn ), Peptostreptococcus anaerobius ( Pa ) and Parvimonas micra ( Pm ) by quantitative PCR in 309 …
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Multidrug-resistant Klebsiella pneumoniae (MRKP) has steadily grown beyond antibiotic control. However, a bacteriophage is considered to be a potential antibiotic alternative for treating bacterial infections. In this study, a lytic bacteriophage, phage 1513, was isolated using a clinical MRKP isolate KP 1513 as the host and was characterized.
Here, we identified a Salmonella T3SS effector, SopF, that potently blocked bacterial autophagy. Removal of sopF from S. Typhimurium led to about 80% intracellular bacteria being targeted by autophagy. We identified the V-ATPase-ATG16L1 axis that initiated xenophagy upon bacteria-induced vacuolar damage. SopF specifically ADP-ribosylated Gln124 of ATP6V0C in the V-ATPase to block its ...
Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates were multidrug-resistant to extensively drug-resistant, exhibiting resistance against 5–7 ...
It has frequently been hypothesized that the helical body shapes of flagellated bacteria may yield some advantage in swimming ability. In particular, the helical-shaped pathogen Helicobacter pylori is often claimed to swim like a corkscrew through its harsh gastric habitat, but there has been no direct confirmation or quantification of such claims. Using fast time-resolution and high ...
Irinotecan is a widely used anticancer pro-drug that is converted in the liver into the active form, but when it gets into the gut, the normally benign microbial flora can convert it into the toxic form, which kills the rapidly multiplying gut epithelium as it would kill rapidly dividing tumor cells, and thus causes diarrhea. Wallace et al. (p.
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